Table 3 Five protocols to extract cannabis by dynamic maceration (Romano and Hazekamp 2013)
From: Processing and extraction methods of medicinal cannabis: a narrative review
| Cannabis (g) | 5 | 5 | 5 | 5 | 10 |
| Solvent (mL) | Naphtha (200) | Petroleum ether (200) | Ethanol (200) | Olive oil (20) + water (70) | Olive oil (100) |
| Extraction/filtration 1 | 5 g Cannabis + 100 mL solvent, agitate 20 min | 5 g Cannabis + 100 mL solvent, agitate 20 min | 5 g Cannabis + 100 mL solvent, agitate 20 min | 5 g Cannabis + 20 mL olive oil + 50 mL water; heat at 100 °C 60 min | 10 g cannabis + 100 mL olive oil; heat at 100 °C 120 min |
| Cool | Cool | ||||
| Paper filtration | Paper filtration | Paper filtration | Paper filtration with pressure | Paper filtration with pressure | |
| Extraction/filtration 2 | 100 mL solvent, agitate 20 min | 100 mL solvent, agitate 20 min | 100 mL solvent, agitate 20 min | 20 mL of hot water | |
| Paper filtration | Paper filtration | Paper filtration | Paper filtration with pressure | - | |
| Combine extracts | Combine extracts | Combine extracts | Combine extracts | ||
| Extract clean up | None | None | Optional activated charcoal filtration | None | None |
| Evaporation/separation | Boiling water bath with nitrogen stream | Boiling water bath with nitrogen stream | Boiling water bath with nitrogen stream | Phase separation; freeze; decant oil phase | None |
| Reconstitution | Reconstitute residue with ethanol to 100 mL | Reconstitute residue with ethanol to 100 mL | Reconstitute residue with ethanol to 100 mL | Collect the oil | |
| Extract concentration (cannabis/solvent) | 5 g/100 mL | 5 g/100 mL | 5 g/100 mL | 5 g/20 mL | 10 g/100 mL |
| Dilution factor for analysis | 20 × | 20 × | 20 × | 100 × | 40 × |
| Final concentration (cannabis/solvent) | 2.5 mg/mL | 2.5 mg/mL | 2.5 mg/mL | 2.5 mg/mL | 2.5 mg/mL |